People who inject drugs (PWID) remain at significantly elevated risk for HIV infection, although testing rates remain very low. This study evaluated the acceptability of HIV self-test kits among PWID using a mixed methods study. 230 PWID engaging with a local (SSP) were provided OraQuick In-Home HIV Test kits and instructions for use. Primary outcomes examined were testing location (onsite vs. home or private location), acceptability (testing self-efficacy), ease of use, reasons and motivations for self-testing, frequency of future testing, and preferences for testing services. Results suggested wide-spread acceptance of this testing method.